Wolf and Twitchy are a wolf-squirrel duo who made their first appearences in Manny, Sid, and Diego's Adventures of Hoodwinked!. They were investigating the Goodie Bandit, the one who stole all of the goodies in the woods.
- Wolf (voiced by Patrick Warburton) is an investigator and the deuteragonist of Manny, Sid, and Diego's Adventures of Hoodwinked!.
- Twitchy (voiced by Cory Edwards) is a squirrel who is Wolf's friend and ally who sometimes say words quickly and act crazy.
- In Manny, Sid, and Diego's Adventures of Balto, they guest starred in this film (along with Kirk Kirkendall) who they helped (along with the Sub-zero Group) Balto lead a group of sled dogs back to the Alaskan city of Nome for medical supplies. At the end, they joined the Sub-zero Group for more further adventures (along with Kirk).